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rb anti ha antibody  (Danaher Inc)


Bioz Verified Symbol Danaher Inc is a verified supplier
Bioz Manufacturer Symbol Danaher Inc manufactures this product  
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    Structured Review

    Danaher Inc rb anti ha antibody
    Rb Anti Ha Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rb+anti+ha/us11958884-211-4-7
    Average 86 stars, based on 1 article reviews
    rb anti ha antibody - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: An impaired ubiquitin-proteasome system increases APOBEC3A abundance
    Article Snippet: Membranes were blocked in 2% ECL prime blocking agent (Cytiva) in TBST (20 mM Tris, 150 mM NaCl, 0.1% Tween 20). .. Membranes were incubated at 4°C in Rb-anti-GAPDH (Proteintech 10494-1-AP; 1:20000), Rb-anti-HA (Abcam ab9110; 1:15000), Rb-anti-alpha tubulin (Abcam ab4074, diluted 1:20000) or Rb-anti-strep (Abcam ab76949, diluted 1:10000) diluted in 1% ECL in TBST at 4°C or room temperature for 12–18 h. Blots were incubated in anti-Rb IgG HRP (Abcam ab97051; 1:20000) in 1% ECL at 4°C for 1.5 h. Blots were developed with ECL prime western blotting detection reagent (Cytiva) before imaging on a Bio-Rad ChemiDoc MP Imaging System using the ‘chemi’ auto exposure or signal accumulation settings. .. For endogenous A3A immunoblotting, cells were lysed in RIPA buffer (150 mM NaCl, 50 mM Tris–HCl, pH 8.0, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, Pierce Protease Inhibitor Tablet, EDTA-free).

    Western Blot:

    Article Title: An impaired ubiquitin-proteasome system increases APOBEC3A abundance
    Article Snippet: Membranes were blocked in 2% ECL prime blocking agent (Cytiva) in TBST (20 mM Tris, 150 mM NaCl, 0.1% Tween 20). .. Membranes were incubated at 4°C in Rb-anti-GAPDH (Proteintech 10494-1-AP; 1:20000), Rb-anti-HA (Abcam ab9110; 1:15000), Rb-anti-alpha tubulin (Abcam ab4074, diluted 1:20000) or Rb-anti-strep (Abcam ab76949, diluted 1:10000) diluted in 1% ECL in TBST at 4°C or room temperature for 12–18 h. Blots were incubated in anti-Rb IgG HRP (Abcam ab97051; 1:20000) in 1% ECL at 4°C for 1.5 h. Blots were developed with ECL prime western blotting detection reagent (Cytiva) before imaging on a Bio-Rad ChemiDoc MP Imaging System using the ‘chemi’ auto exposure or signal accumulation settings. .. For endogenous A3A immunoblotting, cells were lysed in RIPA buffer (150 mM NaCl, 50 mM Tris–HCl, pH 8.0, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, Pierce Protease Inhibitor Tablet, EDTA-free).

    Imaging:

    Article Title: An impaired ubiquitin-proteasome system increases APOBEC3A abundance
    Article Snippet: Membranes were blocked in 2% ECL prime blocking agent (Cytiva) in TBST (20 mM Tris, 150 mM NaCl, 0.1% Tween 20). .. Membranes were incubated at 4°C in Rb-anti-GAPDH (Proteintech 10494-1-AP; 1:20000), Rb-anti-HA (Abcam ab9110; 1:15000), Rb-anti-alpha tubulin (Abcam ab4074, diluted 1:20000) or Rb-anti-strep (Abcam ab76949, diluted 1:10000) diluted in 1% ECL in TBST at 4°C or room temperature for 12–18 h. Blots were incubated in anti-Rb IgG HRP (Abcam ab97051; 1:20000) in 1% ECL at 4°C for 1.5 h. Blots were developed with ECL prime western blotting detection reagent (Cytiva) before imaging on a Bio-Rad ChemiDoc MP Imaging System using the ‘chemi’ auto exposure or signal accumulation settings. .. For endogenous A3A immunoblotting, cells were lysed in RIPA buffer (150 mM NaCl, 50 mM Tris–HCl, pH 8.0, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, Pierce Protease Inhibitor Tablet, EDTA-free).



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